Mouse CD39/ENTPD1 ELISA Kit PicoKine®

Ectonucleoside triphosphate diphosphohydrolase-1 (gene: ENTPD1; protein: NTPDase1) also known as CD39 (Cluster of Differentiation 39), is a typical cell surface-located enzymes with an extracellularly facing catalytic site. This gene is mapped to 19 C3; 19 34.25 cM. NTPDase1 is an ectonucleotidase that catalyse the hydrolysis of γ- and β-phosphate residues of triphospho- and diphosphonucleosides to the monophosphonucleoside derivative. For example, NTPDase1 hydrolyzes P2 receptor ligands, namely ATP, ADP, UTP and UDP with similar efficacy. NTPDase1 can therefore affect P2 receptor activation and functions.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 54 55 56 56 1.87 3.3% Sample 2 215 217 239 228 224 9.60 4.2% Sample 3 956 851 896 882 896 38.14 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 54 55 56 56 1.87 3.3% Sample 1 59 54 55 56 56 1.87 3.3% Sample 2 215 217 239 228 224 9.60 4.2% Sample 2 215 217 239 228 224 9.60 4.2% Sample 3 956 851 896 882 896 38.14 4.2% Sample 3 956 851 896 882 896 38.14 4.2%

Ectonucleoside triphosphate diphosphohydrolase 1; NTPDase 1; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen; CD39; Entpd1; Cd39

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 215 956 60 234 991 Standard deviation 3.01 11.40 53.54 3.96 15.91 70.36 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 215 956 60 234 991 Standard deviation 3.01 11.40 53.54 3.96 15.91 70.36 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 215 956 60 234 991 Mean (pg/ml) 59 215 956 60 234 991 Standard deviation 3.01 11.40 53.54 3.96 15.91 70.36 Standard deviation 3.01 11.40 53.54 3.96 15.91 70.36 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1%

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.051 0.07 0.106 0.207 0.456 1.035 2.035 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.051 0.07 0.106 0.207 0.456 1.035 2.035 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.051 0.07 0.106 0.207 0.456 1.035 2.035 O.D. 0.038 0.051 0.07 0.106 0.207 0.456 1.035 2.035

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.