Mouse SLAMF7 ELISA Kit PicoKine®

SLAM family member 7 is a protein that in humans is encoded by the SLAMF7 gene. This gene is mapped to 1; 1 H3. The surface antigen CD319 (SLAMF7) is a robust marker of normal plasma cells and malignant plasma cells in multiple myeloma. In contrast to CD138 (the traditional plasma cell marker), CD319/SLAMF7 is much more stable and allows robust isolation of malignant plasma cells from delayed or even cryopreserved samples.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 54 47 51 52 4.03 7.7% Sample 2 226 265 248 245 246 13.83 5.6% Sample 3 941 882 888 907 904 23.00 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 54 47 51 52 4.03 7.7% Sample 1 58 54 47 51 52 4.03 7.7% Sample 2 226 265 248 245 246 13.83 5.6% Sample 2 226 265 248 245 246 13.83 5.6% Sample 3 941 882 888 907 904 23.00 2.5% Sample 3 941 882 888 907 904 23.00 2.5%

Expressed in spleen, lymph node, bone marrow and testis. Lower levels detected in thymus. Expressed in NK cells, B-cells, natural killer cells and activated T-cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 226 941 61 231 967 Standard deviation 2.32 9.72 43.29 3.11 12.94 57.05 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 226 941 61 231 967 Standard deviation 2.32 9.72 43.29 3.11 12.94 57.05 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 58 226 941 61 231 967 Mean (pg/ml) 58 226 941 61 231 967 Standard deviation 2.32 9.72 43.29 3.11 12.94 57.05 Standard deviation 2.32 9.72 43.29 3.11 12.94 57.05 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9%

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Mediates natural killer (NK) cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. Positively regulates NK cell functions by a mechanism dependent on the adapter SH2D1B. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells. May play a role in lymphocyte adhesion. In LPS-activated monocytes negatively regulates production of proinflammatory cytokines.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.125 0.179 0.289 0.445 0.856 1.537 2.664 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.125 0.179 0.289 0.445 0.856 1.537 2.664 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.125 0.179 0.289 0.445 0.856 1.537 2.664 O.D. 0.055 0.125 0.179 0.289 0.445 0.856 1.537 2.664

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.