Mouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine®

Chitinase 3-like 3, also known as ECF-L and Ym1, is a secreted 377 amino acid lectin that binds heparin and GlcN oligomers. This gene is mapped to 3 F2.2; 3 46.49 Cm. CHI3L3 is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins, but lacks enzymatic activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 327 332 292 319 317 15.43 4.8% Sample 2 1000 1134 1146 1166 1111 65.38 5.8% Sample 3 4032 3785 4495 4279 4147 265.89 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 327 332 292 319 317 15.43 4.8% Sample 1 327 332 292 319 317 15.43 4.8% Sample 2 1000 1134 1146 1166 1111 65.38 5.8% Sample 2 1000 1134 1146 1166 1111 65.38 5.8% Sample 3 4032 3785 4495 4279 4147 265.89 6.4% Sample 3 4032 3785 4495 4279 4147 265.89 6.4%

Chitinase-like protein 3; Beta-N-acetylhexosaminidase Ym1; Chitinase-3-like protein 3; ECF-L; Eosinophil chemotactic cytokine; Secreted protein Ym1; Chil3; Chi3l3; Ym1

Expressed in peritoneal cavity macrophages and in peritoneal and bone marrow-derived neutrophils. Abundantly expressed in bone marrow, with moderate levels detected in gastric antrum, spleen and in alveolar macrophages in lung. Not detected in brain, heart, liver, kidney, stomach, intestine, skeletal muscle, ovary, testis, thymus and lymph nodes (at protein level) . Detected at low levels in bone marrow, spleen, thymus and lung. Barely detectable in intestine, kidney and coecum.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 327 1000 4032 308 1073 3654 Standard deviation 13.73 67 221.76 18.78 83.69 204.62 CV (%) 4.2% 6.7% 5.5% 6.1% 7.8% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 327 1000 4032 308 1073 3654 Standard deviation 13.73 67 221.76 18.78 83.69 204.62 CV (%) 4.2% 6.7% 5.5% 6.1% 7.8% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 327 1000 4032 308 1073 3654 Mean (pg/ml) 327 1000 4032 308 1073 3654 Standard deviation 13.73 67 221.76 18.78 83.69 204.62 Standard deviation 13.73 67 221.76 18.78 83.69 204.62 CV (%) 4.2% 6.7% 5.5% 6.1% 7.8% 5.6% CV (%) 4.2% 6.7% 5.5% 6.1% 7.8% 5.6%

Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.086 0.158 0.296 0.55 1.001 1.497 1.924 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.086 0.158 0.296 0.55 1.001 1.497 1.924 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.086 0.158 0.296 0.55 1.001 1.497 1.924 O.D. 0.017 0.086 0.158 0.296 0.55 1.001 1.497 1.924

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.