Human SEMA4G ELISA Kit PicoKine®

Human SEMA4G ELISA Kit PicoKine® (96 Tests) . Quantitate Human SEMA4G in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Semaphorin-4G is a protein that in humans is encoded by the SEMA4G gene. This gene is mapped to 10q24.31. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 23 24 24 24 1.50 6.2% Sample 2 102 98 104 102 101 2.17 2.1% Sample 3 468 537 490 506 500 25.14 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 23 24 24 24 1.50 6.2% Sample 1 27 23 24 24 24 1.50 6.2% Sample 2 102 98 104 102 101 2.17 2.1% Sample 2 102 98 104 102 101 2.17 2.1% Sample 3 468 537 490 506 500 25.14 5.0% Sample 3 468 537 490 506 500 25.14 5.0%

Synonyms

Semaphorin-4G; SEMA4G; KIAA1619

Gene Name

SEMA4G

Gene ID

57715

UniProt

Q9NTN9

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V18-L675

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Growth and Development, Neurology Process, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 102 468 29 114 488 Standard deviation 1.24 5.10 24.34 1.48 6.73 31.23 CV (%) 4.6% 5.0% 5.2% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 102 468 29 114 488 Standard deviation 1.24 5.10 24.34 1.48 6.73 31.23 CV (%) 4.6% 5.0% 5.2% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 27 102 468 29 114 488 Mean (pg/ml) 27 102 468 29 114 488 Standard deviation 1.24 5.10 24.34 1.48 6.73 31.23 Standard deviation 1.24 5.10 24.34 1.48 6.73 31.23 CV (%) 4.6% 5.0% 5.2% 5.1% 5.9% 6.4% CV (%) 4.6% 5.0% 5.2% 5.1% 5.9% 6.4%

Sensitivity

<10 pg/mL

Function

Cell surface receptor for PLXNB2. May play a role in axon guidance.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human SEMA4G

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Semaphorin 4G

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Protein Name

Semaphorin-4G

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.