Mouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine®

Mouse TNFRSF23, also known as TNFRH1 and DcTRAIL R1, is a GPI-anchored mouse TNF receptor superfamily member that lacks a cytoplasmic death domain and does not induce apoptosis. This gene is mapped to 7 F5; 7 88.28 Cm. This gene encodes a member of the tumor necrosis factor superfamily of proteins. The encoded receptor has been shown to bind to the ligand TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), but to have no signaling capacity. This gene shows elevated expression in mice with diet-induced fatty liver disease. This gene and other family members are present in a gene cluster on chromosome 7. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 32 37 34 33 2.29 6.9% Sample 2 118 121 115 107 115 5.21 4.5% Sample 3 452 460 462 460 458 3.84 0.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 32 37 34 33 2.29 6.9% Sample 1 31 32 37 34 33 2.29 6.9% Sample 2 118 121 115 107 115 5.21 4.5% Sample 2 118 121 115 107 115 5.21 4.5% Sample 3 452 460 462 460 458 3.84 0.8% Sample 3 452 460 462 460 458 3.84 0.8%

Tumor necrosis factor receptor superfamily member 23; Decoy TRAIL receptor 1; TNF receptor family member SOB; TNF receptor homolog 1; Tumor necrosis factor receptor p60 homolog 1; Tnfrsf23; Dctrailr1; Tnfrh1; Tnfrsf1al1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 118 452 33 124 468 Standard deviation 1.49 5.55 22.15 1.68 6.70 31.36 CV (%) 4.8% 4.7% 4.9% 5.1% 5.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 118 452 33 124 468 Standard deviation 1.49 5.55 22.15 1.68 6.70 31.36 CV (%) 4.8% 4.7% 4.9% 5.1% 5.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 118 452 33 124 468 Mean (pg/ml) 31 118 452 33 124 468 Standard deviation 1.49 5.55 22.15 1.68 6.70 31.36 Standard deviation 1.49 5.55 22.15 1.68 6.70 31.36 CV (%) 4.8% 4.7% 4.9% 5.1% 5.4% 6.7% CV (%) 4.8% 4.7% 4.9% 5.1% 5.4% 6.7%

Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis through ligand competition. Cannot induce the NF-kappa-B pathway.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.