Mouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine®

TNFRSF22 interacts via a complex receptor system to preferentially induce apoptosis of tumor cells in vivo and in vitro. This gene is mapped to 7 F5; 7 88.27 cM. The mouse decoy TRAIL R2 (TNFRSF22) gene encodes a 198 amino acid (aa) residues type I transmembrane protein with a putative 39 aa signal peptide, a 136 aa extracellular domain, a 18 aa transmembrane region, and a 5 aa cytoplasmic tail. An alternatively spliced variant encoding a secreted soluble receptor of 180 aa has also been described. The transmembrane and soluble receptors are known as mDcTRAIL R2L and mDcTRAIL R2S, respectively. Both mDcTRAIL R2 isoforms and the GPI-linked mDcTRAIL R1 bind TRAIL specifically but are incapable of triggering signal transduction. Mouse DcTRAIL R1 and mDcTRAIL R2 share 71% sequence identity. The two receptors have similar structural modules in their CRDs, which differ from those found in the CRDs of mTNF RH3. Mouse DcTRAIL R1 preferentially binds mTRAIL while mDcTRAIL R2 binds both human and mTRAIL. mRNA of mouse DcTRAIL R1 and -2 are expressed at low levels in many mouse tissues and at higher levels in several mouse cell lines.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 34 31 32 31 1.80 5.8% Sample 2 102 105 105 112 106 3.67 3.4% Sample 3 469 508 495 507 494 15.72 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 34 31 32 31 1.80 5.8% Sample 1 29 34 31 32 31 1.80 5.8% Sample 2 102 105 105 112 106 3.67 3.4% Sample 2 102 105 105 112 106 3.67 3.4% Sample 3 469 508 495 507 494 15.72 3.1% Sample 3 469 508 495 507 494 15.72 3.1%

Tumor necrosis factor receptor superfamily member 22; Decoy TRAIL receptor 2; TNF receptor family member SOBa; TNF receptor homolog 2; Tumor necrosis factor receptor p60 homolog 2; Tnfrsf22; Dctrailr2; Tnfrh2; Tnfrsf1al2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 102 469 31 116 475 Standard deviation 1.42 5.41 25.33 2.05 7.89 33.25 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 102 469 31 116 475 Standard deviation 1.42 5.41 25.33 2.05 7.89 33.25 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 102 469 31 116 475 Mean (pg/ml) 29 102 469 31 116 475 Standard deviation 1.42 5.41 25.33 2.05 7.89 33.25 Standard deviation 1.42 5.41 25.33 2.05 7.89 33.25 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.0% CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.0%

Receptor for the cytotoxic ligand TNFSF10/TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. Protects cells against TRAIL mediated apoptosis possibly through ligand competition. Cannot induce the NF-kappa-B pathway.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.