Human CTRC/Chymotrypsin-C ELISA Kit PicoKine®

Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. This gene is mapped to 1p36.21. This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 229 278 272 238 254 21.09 8.3% Sample 2 1188 1214 1275 1228 1226 31.59 2.5% Sample 3 4782 5075 5245 4924 5006 172.32 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 229 278 272 238 254 21.09 8.3% Sample 1 229 278 272 238 254 21.09 8.3% Sample 2 1188 1214 1275 1228 1226 31.59 2.5% Sample 2 1188 1214 1275 1228 1226 31.59 2.5% Sample 3 4782 5075 5245 4924 5006 172.32 3.4% Sample 3 4782 5075 5245 4924 5006 172.32 3.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 229 1188 4782 314 1249 4914 Standard deviation 12.14 66.53 277.36 20.72 86.18 358.72 CV (%) 5.3% 5.6% 5.8% 6.6% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 229 1188 4782 314 1249 4914 Standard deviation 12.14 66.53 277.36 20.72 86.18 358.72 CV (%) 5.3% 5.6% 5.8% 6.6% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 229 1188 4782 314 1249 4914 Mean (pg/ml) 229 1188 4782 314 1249 4914 Standard deviation 12.14 66.53 277.36 20.72 86.18 358.72 Standard deviation 12.14 66.53 277.36 20.72 86.18 358.72 CV (%) 5.3% 5.6% 5.8% 6.6% 6.9% 7.3% CV (%) 5.3% 5.6% 5.8% 6.6% 6.9% 7.3%

Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.153 0.25 0.389 0.649 1.033 1.857 2.832 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.153 0.25 0.389 0.649 1.033 1.857 2.832 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.153 0.25 0.389 0.649 1.033 1.857 2.832 O.D. 0.031 0.153 0.25 0.389 0.649 1.033 1.857 2.832

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.