Mouse AFP ELISA Kit PicoKine®

Mouse AFP ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Afp in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 787 751 817 732 771 32.75 4.2% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 3 9095 10188 9364 8739 9346 534.02 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 787 751 817 732 771 32.75 4.2% Sample 1 787 751 817 732 771 32.75 4.2% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 3 9095 10188 9364 8739 9346 534.02 5.7% Sample 3 9095 10188 9364 8739 9346 534.02 5.7%

Synonyms

Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Afp

Gene Name

Afp

Gene ID

11576

UniProt

P02772

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K19-V605

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Plasma.

Applications

ELISA

Field of Research

Cancer, Cardiovascular, Developmental Biology, Organogenesis, Serum Proteins, Tumor Antigens, Tumor Biomarkers

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 9095 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 5.6% 6.3% 4.8% 6.2% 6.6% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 9095 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 5.6% 6.3% 4.8% 6.2% 6.6% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 9095 804 2193 8576 Mean (pg/ml) 787 2378 9095 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 5.6% 6.3% 4.8% 6.2% 6.6% 5.5% CV (%) 5.6% 6.3% 4.8% 6.2% 6.6% 5.5%

Sensitivity

<10 pg/mL

Function

Binds estrogens, fatty acids and metals.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse AFP

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Alpha fetoprotein

Subcellular Location

Secreted.

Protein Name

Alpha-fetoprotein

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.086 0.122 0.175 0.276 0.52 1.052 1.908 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.086 0.122 0.175 0.276 0.52 1.052 1.908 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.086 0.122 0.175 0.276 0.52 1.052 1.908 O.D. 0.032 0.086 0.122 0.175 0.276 0.52 1.052 1.908

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.