Mouse AFP ELISA Kit PicoKine®

Alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 787 751 817 732 771 32.75 4.2% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 3 9095 10188 9364 8739 9346 534.02 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 787 751 817 732 771 32.75 4.2% Sample 1 787 751 817 732 771 32.75 4.2% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 2 2378 2440 2261 2414 2373 68.44 2.8% Sample 3 9095 10188 9364 8739 9346 534.02 5.7% Sample 3 9095 10188 9364 8739 9346 534.02 5.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 9095 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 5.6% 6.3% 4.8% 6.2% 6.6% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 9095 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 5.6% 6.3% 4.8% 6.2% 6.6% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 787 2378 9095 804 2193 8576 Mean (pg/ml) 787 2378 9095 804 2193 8576 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 Standard deviation 44.07 149.81 436.56 49.84 144.73 471.68 CV (%) 5.6% 6.3% 4.8% 6.2% 6.6% 5.5% CV (%) 5.6% 6.3% 4.8% 6.2% 6.6% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.086 0.122 0.175 0.276 0.52 1.052 1.908 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.086 0.122 0.175 0.276 0.52 1.052 1.908 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.086 0.122 0.175 0.276 0.52 1.052 1.908 O.D. 0.032 0.086 0.122 0.175 0.276 0.52 1.052 1.908

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.