Mouse CHIT1 ELISA Kit PicoKine®

Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1; 1 E4. It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract. CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice. Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form. Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease. The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme. Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 62 36 48 51 3.33 6.6% Sample 2 100 80 124 79 96 6.81 7.1% Sample 3 930 789 781 644 786 32.3 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 62 36 48 51 3.33 6.6% Sample 1 57 62 36 48 51 3.33 6.6% Sample 2 100 80 124 79 96 6.81 7.1% Sample 2 100 80 124 79 96 6.81 7.1% Sample 3 930 789 781 644 786 32.3 4.1% Sample 3 930 789 781 644 786 32.3 4.1%

Highly expressed in tongue, stomach, kidney, brain, skin, testis, and bone marrow. Low level of expression was found in lung, heart, spleen, small intestine, and liver. Not detectable in pancreas, salivary gland, large intestine, uterus, or peripheral blood mononuclear cells (PBMC) .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 100 930 51 66 628 Standard deviation 2.98 7.3 68.82 1.55 3.93 42.77 CV (%) 5.2% 7.3% 7.4% 3% 6% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 100 930 51 66 628 Standard deviation 2.98 7.3 68.82 1.55 3.93 42.77 CV (%) 5.2% 7.3% 7.4% 3% 6% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 100 930 51 66 628 Mean (pg/ml) 57 100 930 51 66 628 Standard deviation 2.98 7.3 68.82 1.55 3.93 42.77 Standard deviation 2.98 7.3 68.82 1.55 3.93 42.77 CV (%) 5.2% 7.3% 7.4% 3% 6% 6.8% CV (%) 5.2% 7.3% 7.4% 3% 6% 6.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.185 0.279 0.403 0.669 1.015 1.557 2.329 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.185 0.279 0.403 0.669 1.015 1.557 2.329 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.185 0.279 0.403 0.669 1.015 1.557 2.329 O.D. 0.009 0.185 0.279 0.403 0.669 1.015 1.557 2.329

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.