Human TPP1/Tripeptidyl-peptidase 1 ELISA Kit PicoKine®

Tripeptidyl-peptidase 1, also known as Lysosomal pepstatin-insensitive protease, is an enzyme that in humans is encoded by the TPP1 gene. The human gene TPP1 encodes a member of the sedolisin family of serine proteases. The human gene has 13 exons and locates at the chromosome band 11p15. This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 288 285 315 290 15.63 5.3% Sample 2 1160 1088 1127 980 1088 67.76 6.2% Sample 3 3946 4041 3931 4546 4116 251.81 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 288 285 315 290 15.63 5.3% Sample 1 272 288 285 315 290 15.63 5.3% Sample 2 1160 1088 1127 980 1088 67.76 6.2% Sample 2 1160 1088 1127 980 1088 67.76 6.2% Sample 3 3946 4041 3931 4546 4116 251.81 6.1% Sample 3 3946 4041 3931 4546 4116 251.81 6.1%

Tripeptidyl-peptidase 1; TPP-1; Cell growth-inhibiting gene 1 protein; Lysosomal pepstatin-insensitive protease; LPIC; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase I; TPP-I; TPP1; CLN2; GIG1; UNQ267; PRO304

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1160 3946 303 1187 4281 Standard deviation 10.88 54.52 205.19 15.45 70.03 303.95 CV (%) 4.0% 4.7% 5.2% 5.1% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1160 3946 303 1187 4281 Standard deviation 10.88 54.52 205.19 15.45 70.03 303.95 CV (%) 4.0% 4.7% 5.2% 5.1% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1160 3946 303 1187 4281 Mean (pg/ml) 272 1160 3946 303 1187 4281 Standard deviation 10.88 54.52 205.19 15.45 70.03 303.95 Standard deviation 10.88 54.52 205.19 15.45 70.03 303.95 CV (%) 4.0% 4.7% 5.2% 5.1% 5.9% 7.1% CV (%) 4.0% 4.7% 5.2% 5.1% 5.9% 7.1%

Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.107 0.207 0.371 0.729 1.374 2.134 2.35 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.107 0.207 0.371 0.729 1.374 2.134 2.35 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.107 0.207 0.371 0.729 1.374 2.134 2.35 O.D. 0.004 0.107 0.207 0.371 0.729 1.374 2.134 2.35

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.