Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®

Human TCN2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human TCN2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 292 319 286 295 13.82 4.6% Sample 2 1139 1247 1212 1220 1204 39.97 3.3% Sample 3 5792 6174 5259 6098 5830 359.73 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 292 319 286 295 13.82 4.6% Sample 1 285 292 319 286 295 13.82 4.6% Sample 2 1139 1247 1212 1220 1204 39.97 3.3% Sample 2 1139 1247 1212 1220 1204 39.97 3.3% Sample 3 5792 6174 5259 6098 5830 359.73 6.1% Sample 3 5792 6174 5259 6098 5830 359.73 6.1%

Synonyms

Transcobalamin-2; TC-2; Transcobalamin II; TC II; TCII; TCN2; TC2

Gene Name

TCN2

Gene ID

6948

UniProt

P20062

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-W427

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Cofactors, Vitamins/Minerals, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction, Vitamins/Minerals

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1139 5792 1123 1123 5755 Standard deviation 17.38 72.89 457.56 18.45 74.11 552.48 CV (%) 6.1% 6.4% 7.9% 6.6% 6.6% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1139 5792 1123 1123 5755 Standard deviation 17.38 72.89 457.56 18.45 74.11 552.48 CV (%) 6.1% 6.4% 7.9% 6.6% 6.6% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 285 1139 5792 1123 1123 5755 Mean (pg/ml) 285 1139 5792 1123 1123 5755 Standard deviation 17.38 72.89 457.56 18.45 74.11 552.48 Standard deviation 17.38 72.89 457.56 18.45 74.11 552.48 CV (%) 6.1% 6.4% 7.9% 6.6% 6.6% 9.6% CV (%) 6.1% 6.4% 7.9% 6.6% 6.6% 9.6%

Sensitivity

<50 pg/mL

Function

Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human TCN2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Transcobalamin 2

Subcellular Location

Secreted.

Protein Name

Transcobalamin-2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.046 0.104 0.244 0.517 0.931 1.546 1.938 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.046 0.104 0.244 0.517 0.931 1.546 1.938 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.046 0.104 0.244 0.517 0.931 1.546 1.938 O.D. 0.002 0.046 0.104 0.244 0.517 0.931 1.546 1.938

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.