Human ANGPTL7 ELISA Kit PicoKine®

Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 1p36.22. Angiopoietin-like Protein 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 45?50 kDa glycoprotein member of the angiopoietin?like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. Its production is up?regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. ANGPTL7 expression is up?regulated under conditions of increased ocular pressure.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 129 118 129 129 7.77 6.0% Sample 2 644 660 702 583 647 42.71 6.6% Sample 3 1907 2152 2078 2215 2088 115.20 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 129 118 129 129 7.77 6.0% Sample 1 140 129 118 129 129 7.77 6.0% Sample 2 644 660 702 583 647 42.71 6.6% Sample 2 644 660 702 583 647 42.71 6.6% Sample 3 1907 2152 2078 2215 2088 115.20 5.5% Sample 3 1907 2152 2078 2215 2088 115.20 5.5%

Angiopoietin-related protein 7; Angiopoietin-like factor; Angiopoietin-like protein 7; Cornea-derived transcript 6 protein; ANGPTL7; CDT6; UNQ313; PRO356

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.