Human Myeloblastin ELISA Kit PicoKine®

Human Myeloblastin ELISA Kit PicoKine® (96 Tests) . Quantitate Human PRTN3 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Proteinase 3 also known as PRTN3 is an enzyme that in humans is encoded by the PRTN3 gene. This gene is mapped to 19p13.3. PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown, but, in human neutrophils, proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the target of anti-neutrophil cytoplasmic antibodies (ANCAs) of the c-ANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease granulomatosis with polyangiitis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 115 106 113 110 3.49 3.1% Sample 2 326 345 331 317 329 10.13 3.0% Sample 3 1513 1345 1372 1433 1415 64.56 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 115 106 113 110 3.49 3.1% Sample 1 109 115 106 113 110 3.49 3.1% Sample 2 326 345 331 317 329 10.13 3.0% Sample 2 326 345 331 317 329 10.13 3.0% Sample 3 1513 1345 1372 1433 1415 64.56 4.5% Sample 3 1513 1345 1372 1433 1415 64.56 4.5%

Synonyms

Myeloblastin; AGP7; C-ANCA antigen; Leukocyte proteinase 3; PR-3; PR3; Neutrophil proteinase 4; NP-4; P29; Wegener autoantigen; PRTN3; MBN

Gene Name

PRTN3

Gene ID

5657

UniProt

P24158

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I28-R248

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in polymorphonuclear leukocytes (at protein level) . Expressed in neutrophils (at protein level) . Expressed in differentiating neutrophils.

Applications

ELISA

Field of Research

Collagen, Cytoskeleton/ECM, ECM Enzymes, ECM Proteins, Extracellular Matrix, Signal Transduction

Standard

4 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 326 1513 116 401 1765 Standard deviation 4.58 14.02 62.03 7.66 27.27 125.32 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 326 1513 116 401 1765 Standard deviation 4.58 14.02 62.03 7.66 27.27 125.32 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 109 326 1513 116 401 1765 Mean (pg/ml) 109 326 1513 116 401 1765 Standard deviation 4.58 14.02 62.03 7.66 27.27 125.32 Standard deviation 4.58 14.02 62.03 7.66 27.27 125.32 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1%

Sensitivity

<10 pg/mL

Function

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) . By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Myeloblastin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Proteinase 3

Subcellular Location

Secreted. Cell membrane. Peripheral membrane protein. Extracellular side. Cytoplasmic granule. Membrane raft. Peripheral membrane protein. Extracellular side.

Protein Name

Myeloblastin

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.707 2.129 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.707 2.129 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.707 2.129 O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.707 2.129

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.