Human Chitotriosidase/CHIT1 ELISA Kit PicoKine®

Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1q32.1. Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 610 690 615 45.06 7.3% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 3 7933 8323 9098 7778 8283 510.72 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 610 690 615 45.06 7.3% Sample 1 590 572 610 690 615 45.06 7.3% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 2 1670 1703 1941 1771 1771 104.55 5.9% Sample 3 7933 8323 9098 7778 8283 510.72 6.1% Sample 3 7933 8323 9098 7778 8283 510.72 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6%

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.557 2.129 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.557 2.129 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.557 2.129 O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.557 2.129

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.