Rat Matrilin-3 ELISA Kit PicoKine®

Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 6q14. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 249 217 254 241 14.51 6.0% Sample 2 1165 1151 1031 1075 1105 54.97 4.9% Sample 3 4441 5053 4576 4552 4655 235.07 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 249 217 254 241 14.51 6.0% Sample 1 247 249 217 254 241 14.51 6.0% Sample 2 1165 1151 1031 1075 1105 54.97 4.9% Sample 2 1165 1151 1031 1075 1105 54.97 4.9% Sample 3 4441 5053 4576 4552 4655 235.07 5.0% Sample 3 4441 5053 4576 4552 4655 235.07 5.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1165 4441 303 1139 4756 Standard deviation 12.10 50.10 182.08 18.18 66.06 328.16 CV (%) 4.9% 4.3% 4.1% 6.0% 5.8% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1165 4441 303 1139 4756 Standard deviation 12.10 50.10 182.08 18.18 66.06 328.16 CV (%) 4.9% 4.3% 4.1% 6.0% 5.8% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 1165 4441 303 1139 4756 Mean (pg/ml) 247 1165 4441 303 1139 4756 Standard deviation 12.10 50.10 182.08 18.18 66.06 328.16 Standard deviation 12.10 50.10 182.08 18.18 66.06 328.16 CV (%) 4.9% 4.3% 4.1% 6.0% 5.8% 6.9% CV (%) 4.9% 4.3% 4.1% 6.0% 5.8% 6.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.145 0.219 0.386 0.711 1.157 1.728 2.115 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.145 0.219 0.386 0.711 1.157 1.728 2.115 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.145 0.219 0.386 0.711 1.157 1.728 2.115 O.D. 0.072 0.145 0.219 0.386 0.711 1.157 1.728 2.115

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.