Mouse Matrilin-3 ELISA Kit PicoKine®

Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 12 A1.1; 12 3.96 cM. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 227 212 214 224 219 6.37 2.9% Sample 2 983 926 1041 1054 1001 50.88 5.0% Sample 3 3812 4144 3641 3647 3811 204.13 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 227 212 214 224 219 6.37 2.9% Sample 1 227 212 214 224 219 6.37 2.9% Sample 2 983 926 1041 1054 1001 50.88 5.0% Sample 2 983 926 1041 1054 1001 50.88 5.0% Sample 3 3812 4144 3641 3647 3811 204.13 5.3% Sample 3 3812 4144 3641 3647 3811 204.13 5.3%

Strongly expressed in growing skeletal tissue such as epiphyseal growth plate or in bone undergoing growth and remodeling. In the bone, actively synthesized in osteoblasts and osteocytes. Expressed in cartilage of sternum, femur, vertebrae, trachea, articular and epiphyseal cartilage, cartilage of developing bones and bones.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 227 983 3812 268 1187 4219 Standard deviation 10.90 48.17 175.35 15.01 81.90 270.02 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 227 983 3812 268 1187 4219 Standard deviation 10.90 48.17 175.35 15.01 81.90 270.02 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 227 983 3812 268 1187 4219 Mean (pg/ml) 227 983 3812 268 1187 4219 Standard deviation 10.90 48.17 175.35 15.01 81.90 270.02 Standard deviation 10.90 48.17 175.35 15.01 81.90 270.02 CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4% CV (%) 4.8% 4.9% 4.6% 5.6% 6.9% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.146 0.217 0.387 0.711 1.157 1.718 2.114 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.146 0.217 0.387 0.711 1.157 1.718 2.114 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.146 0.217 0.387 0.711 1.157 1.718 2.114 O.D. 0.072 0.146 0.217 0.387 0.711 1.157 1.718 2.114

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.