Mouse ERAP1 ELISA Kit PicoKine®

Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator, also known as endoplasmic reticulum aminopeptidase 1 (ARTS-1), is a protein which in humans is encoded by the ARTS-1 gene. This gene is mapped to 13; 13 C1. Endoplasmic reticulum amino peptidase 1 is active in the endoplasmic reticulum, which is involved in protein processing and transport. This protein is an aminopeptidase, which is an enzyme that cleaves peptides at the N-terminal into smaller fragments called amino acids.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 505 578 524 604 552 39.90 7.2% Sample 2 1975 2096 1918 1952 1985 67.07 3.3% Sample 3 8963 10445 9390 9719 9629 541.90 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 505 578 524 604 552 39.90 7.2% Sample 1 505 578 524 604 552 39.90 7.2% Sample 2 1975 2096 1918 1952 1985 67.07 3.3% Sample 2 1975 2096 1918 1952 1985 67.07 3.3% Sample 3 8963 10445 9390 9719 9629 541.90 5.6% Sample 3 8963 10445 9390 9719 9629 541.90 5.6%

Endoplasmic reticulum aminopeptidase 1; ARTS-1; Adipocyte-derived leucine aminopeptidase; A-LAP; Aminopeptidase PILS; Puromycin-insensitive leucyl-specific aminopeptidase; PILS-AP; VEGF-induced aminopeptidase; Erap1; Appils; Arts1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 505 1975 8963 2058 587 9289 Standard deviation 29.29 118.50 412.30 125.54 40.50 678.10 CV (%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 505 1975 8963 2058 587 9289 Standard deviation 29.29 118.50 412.30 125.54 40.50 678.10 CV (%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 505 1975 8963 2058 587 9289 Mean (pg/ml) 505 1975 8963 2058 587 9289 Standard deviation 29.29 118.50 412.30 125.54 40.50 678.10 Standard deviation 29.29 118.50 412.30 125.54 40.50 678.10 CV (%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3% CV (%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3%

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.083 0.132 0.186 0.31 0.586 1.072 1.846 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.083 0.132 0.186 0.31 0.586 1.072 1.846 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.083 0.132 0.186 0.31 0.586 1.072 1.846 O.D. 0.048 0.083 0.132 0.186 0.31 0.586 1.072 1.846

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.