Mouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine®

Mouse Osteoadherin ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Omd in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 13; 13 A5. Osteoadherin is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 279 280 286 293 284 5.59 1.9% Sample 2 1140 983 1062 973 1039 67.49 6.4% Sample 3 4460 4445 3951 4475 4332 220.65 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 279 280 286 293 284 5.59 1.9% Sample 1 279 280 286 293 284 5.59 1.9% Sample 2 1140 983 1062 973 1039 67.49 6.4% Sample 2 1140 983 1062 973 1039 67.49 6.4% Sample 3 4460 4445 3951 4475 4332 220.65 5.0% Sample 3 4460 4445 3951 4475 4332 220.65 5.0%

Synonyms

Osteomodulin; Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; Osteoadherin; OSAD; Omd

Gene Name

Omd

Gene ID

27047

UniProt

O35103

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-I423

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Bone specific.

Applications

ELISA

Field of Research

Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction, Structures

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 279 1140 4460 308 1190 4553 Standard deviation 13.39 49.02 182.86 20.33 69.02 346.03 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 279 1140 4460 308 1190 4553 Standard deviation 13.39 49.02 182.86 20.33 69.02 346.03 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 279 1140 4460 308 1190 4553 Mean (pg/ml) 279 1140 4460 308 1190 4553 Standard deviation 13.39 49.02 182.86 20.33 69.02 346.03 Standard deviation 13.39 49.02 182.86 20.33 69.02 346.03 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6%

Sensitivity

<50 pg/mL

Function

May be implicated in biomineralization processes. Has a function in binding of osteoblasts via the alpha (V) beta3-integrin.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Osteoadherin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Osteomodulin

Subcellular Location

Extracellular matrix.

Protein Name

Osteomodulin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.147 0.243 0.407 0.703 1.145 1.814 2.25 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.147 0.243 0.407 0.703 1.145 1.814 2.25 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.147 0.243 0.407 0.703 1.145 1.814 2.25 O.D. 0.038 0.147 0.243 0.407 0.703 1.145 1.814 2.25

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.