Mouse APOH ELISA Kit PicoKine®

Apolipoprotein H (Apo-H), previously known as β2-glycoprotein I and beta-2 glycoprotein I, is a 38 kDa multifunctional apolipoprotein that in humans is encoded by the APOH gene. This gene is mapped to 11 E1; 11 71.8 Cm. Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 376 379 381 366 375 5.76 1.5% Sample 2 2458 2675 2677 2428 2559 116.98 4.5% Sample 3 8756 9905 9479 8379 9129 597.13 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 376 379 381 366 375 5.76 1.5% Sample 1 376 379 381 366 375 5.76 1.5% Sample 2 2458 2675 2677 2428 2559 116.98 4.5% Sample 2 2458 2675 2677 2428 2559 116.98 4.5% Sample 3 8756 9905 9479 8379 9129 597.13 6.5% Sample 3 8756 9905 9479 8379 9129 597.13 6.5%

Beta-2-glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Apolipoprotein H; Apo-H; Beta-2-glycoprotein I; B2GPI; Beta (2) GPI; Apoh; B2gp1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 376 2458 8756 384 2487 8819 Standard deviation 15.04 140.11 402.78 19.58 171.60 617.33 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 376 2458 8756 384 2487 8819 Standard deviation 15.04 140.11 402.78 19.58 171.60 617.33 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 376 2458 8756 384 2487 8819 Mean (pg/ml) 376 2458 8756 384 2487 8819 Standard deviation 15.04 140.11 402.78 19.58 171.60 617.33 Standard deviation 15.04 140.11 402.78 19.58 171.60 617.33 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0%

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.144 0.245 0.468 0.821 1.315 1.908 2.241 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.144 0.245 0.468 0.821 1.315 1.908 2.241 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.144 0.245 0.468 0.821 1.315 1.908 2.241 O.D. 0.036 0.144 0.245 0.468 0.821 1.315 1.908 2.241

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.