Human SMPD1 ELISA Kit PicoKine®

Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), is an enzyme that in humans is encoded by the SMPD1 gene. This gene is mapped to 11p15.4. The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB) . Multiple transcript variants encoding different isoforms have been identified. Sphingomyelin phosphodiesterase 1 belongs to the sphingomyelin phosphodiesterase family.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 330 332 308 305 319 15.3 4.8% Sample 2 1190 1185 1201 1218 1199 43.15 0.9% Sample 3 4173 4545 4622 4796 4534 231.23 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 330 332 308 305 319 15.3 4.8% Sample 1 330 332 308 305 319 15.3 4.8% Sample 2 1190 1185 1201 1218 1199 43.15 0.9% Sample 2 1190 1185 1201 1218 1199 43.15 0.9% Sample 3 4173 4545 4622 4796 4534 231.23 5.1% Sample 3 4173 4545 4622 4796 4534 231.23 5.1%

Apoptosis, Associated Proteins, Cancer, Cell Biology, Intracellular, Lipid Metabolism, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 330 1190 4173 351 1215 4524 Standard deviation 14.19 61.88 191.96 21.41 71.69 334.78 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 330 1190 4173 351 1215 4524 Standard deviation 14.19 61.88 191.96 21.41 71.69 334.78 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 330 1190 4173 351 1215 4524 Mean (pg/ml) 330 1190 4173 351 1215 4524 Standard deviation 14.19 61.88 191.96 21.41 71.69 334.78 Standard deviation 14.19 61.88 191.96 21.41 71.69 334.78 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.075 0.144 0.245 0.468 0.821 1.315 1.908 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.075 0.144 0.245 0.468 0.821 1.315 1.908 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.075 0.144 0.245 0.468 0.821 1.315 1.908 O.D. 0.036 0.075 0.144 0.245 0.468 0.821 1.315 1.908

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.