Human Matrilin-3 ELISA Kit PicoKine®

Human Matrilin-3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human MATN3 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 2p24.1. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 125 143 138 129 133 7.11 5.3% Sample 2 689 800 745 802 759 46.43 6.1% Sample 3 2209 2281 2219 2542 2312 135.20 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 125 143 138 129 133 7.11 5.3% Sample 1 125 143 138 129 133 7.11 5.3% Sample 2 689 800 745 802 759 46.43 6.1% Sample 2 689 800 745 802 759 46.43 6.1% Sample 3 2209 2281 2219 2542 2312 135.20 5.8% Sample 3 2209 2281 2219 2542 2312 135.20 5.8%

Synonyms

Matrilin-3; MATN3

Gene Name

MATN3

Gene ID

4148

UniProt

O15232

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D29-R486

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed only in cartilaginous tissues, such as vertebrae, ribs and shoulders.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

93.7 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 125 689 2209 148 697 2553 Standard deviation 6.13 29.63 90.57 9.77 40.43 194.03 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 125 689 2209 148 697 2553 Standard deviation 6.13 29.63 90.57 9.77 40.43 194.03 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 125 689 2209 148 697 2553 Mean (pg/ml) 125 689 2209 148 697 2553 Standard deviation 6.13 29.63 90.57 9.77 40.43 194.03 Standard deviation 6.13 29.63 90.57 9.77 40.43 194.03 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6%

Sensitivity

<50 pg/mL

Function

Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Matrilin-3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Matrilin 3

Subcellular Location

Secreted.

Protein Name

Matrilin-3

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.077 0.159 0.319 0.638 1.05 1.594 1.887 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.077 0.159 0.319 0.638 1.05 1.594 1.887 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.077 0.159 0.319 0.638 1.05 1.594 1.887 O.D. 0.032 0.077 0.159 0.319 0.638 1.05 1.594 1.887

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.