Human Matrilin-2 ELISA Kit PicoKine®

Human Matrilin-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human MATN2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Matrilin-2 is a protein that in humans is encoded by the MATN2 gene. This gene is mapped to 8q22.1-q22.2. This gene encodes a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains five von Willebrand factor A domains. The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 34 37 41 38 2.94 7.7% Sample 2 183 175 170 188 179 6.96 3.8% Sample 3 654 569 621 671 628 38.89 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 34 37 41 38 2.94 7.7% Sample 1 41 34 37 41 38 2.94 7.7% Sample 2 183 175 170 188 179 6.96 3.8% Sample 2 183 175 170 188 179 6.96 3.8% Sample 3 654 569 621 671 628 38.89 6.1% Sample 3 654 569 621 671 628 38.89 6.1%

Synonyms

Matrilin-2; MATN2; UNQ193; PRO219

Gene Name

MATN2

Gene ID

4147

UniProt

O00339

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R24-R956

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 183 654 49 212 719 Standard deviation 1.97 10.43 30.08 2.50 12.51 46.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 183 654 49 212 719 Standard deviation 1.97 10.43 30.08 2.50 12.51 46.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 41 183 654 49 212 719 Mean (pg/ml) 41 183 654 49 212 719 Standard deviation 1.97 10.43 30.08 2.50 12.51 46.02 Standard deviation 1.97 10.43 30.08 2.50 12.51 46.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4%

Sensitivity

<10 pg/mL

Function

Involved in matrix assembly.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Matrilin-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Matrilin 2

Subcellular Location

Secreted.

Protein Name

Matrilin-2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.039 0.2 0.292 0.447 0.754 1.101 1.776 2.794 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.039 0.2 0.292 0.447 0.754 1.101 1.776 2.794 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.039 0.2 0.292 0.447 0.754 1.101 1.776 2.794 O.D. 0.039 0.2 0.292 0.447 0.754 1.101 1.776 2.794

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.