Human Brevican ELISA Kit PicoKine®

Brevican core protein is a protein that in humans is encoded by the BCAN gene. This gene is mapped to 1q23.1. Brevican is a member of the lectican protein family. Brevican is localised to the surface of neurons in the brain. In melanocytic cells, BCAN gene expression may be regulated by MITF. This gene encodes a member of the lectican family of chondroitin sulfate proteoglycans that is specifically expressed in the central nervous system. This protein is developmentally regulated and may function in the formation of the brain extracellular matrix. This protein is highly expressed in gliomas and may promote the growth and cell motility of brain tumor cells. Alternate splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 50 49 55 51 2.38 4.6% Sample 2 208 199 203 231 210 12.39 5.9% Sample 3 824 819 818 777 809 18.90 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 50 49 55 51 2.38 4.6% Sample 1 53 50 49 55 51 2.38 4.6% Sample 2 208 199 203 231 210 12.39 5.9% Sample 2 208 199 203 231 210 12.39 5.9% Sample 3 824 819 818 777 809 18.90 2.3% Sample 3 824 819 818 777 809 18.90 2.3%

Alzheimer's Disease, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Neurodegenerative Disease, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 208 824 57 214 958 Standard deviation 2.23 8.94 33.78 3.76 14.55 72.81 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 208 824 57 214 958 Standard deviation 2.23 8.94 33.78 3.76 14.55 72.81 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 208 824 57 214 958 Mean (pg/ml) 53 208 824 57 214 958 Standard deviation 2.23 8.94 33.78 3.76 14.55 72.81 Standard deviation 2.23 8.94 33.78 3.76 14.55 72.81 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.6% CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.6%

May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.039 0.217 0.322 0.507 0.754 1.205 1.776 2.694 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.039 0.217 0.322 0.507 0.754 1.205 1.776 2.694 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.039 0.217 0.322 0.507 0.754 1.205 1.776 2.694 O.D. 0.039 0.217 0.322 0.507 0.754 1.205 1.776 2.694

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.