Human Osteoadherin/OSAD/OMD ELISA Kit PicoKine®

Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 9q22.31. Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP) . LRR motifs consist of approximately 20?30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix. Human OSAD is expressed as an even larger 110 kDa molecule in teeth. OSAD contains eight sulfated tyrosine residues and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C?terminal acidic domain. OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 178 162 182 159 170 9.9 5.8% Sample 2 606 547 628 536 579 38.73 6.6% Sample 3 1031 1040 1142 1024 1059 48.11 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 178 162 182 159 170 9.9 5.8% Sample 1 178 162 182 159 170 9.9 5.8% Sample 2 606 547 628 536 579 38.73 6.6% Sample 2 606 547 628 536 579 38.73 6.6% Sample 3 1031 1040 1142 1024 1059 48.11 4.5% Sample 3 1031 1040 1142 1024 1059 48.11 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 178 606 1031 175 567 1093 Standard deviation 9.79 39.99 75.26 9.62 38.55 96.18 CV (%) 5.5% 6.6% 7.3% 5.5% 6.8% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 178 606 1031 175 567 1093 Standard deviation 9.79 39.99 75.26 9.62 38.55 96.18 CV (%) 5.5% 6.6% 7.3% 5.5% 6.8% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 178 606 1031 175 567 1093 Mean (pg/ml) 178 606 1031 175 567 1093 Standard deviation 9.79 39.99 75.26 9.62 38.55 96.18 Standard deviation 9.79 39.99 75.26 9.62 38.55 96.18 CV (%) 5.5% 6.6% 7.3% 5.5% 6.8% 8.8% CV (%) 5.5% 6.6% 7.3% 5.5% 6.8% 8.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.165 0.231 0.373 0.588 0.93 1.489 2.053 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.165 0.231 0.373 0.588 0.93 1.489 2.053 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.165 0.231 0.373 0.588 0.93 1.489 2.053 O.D. 0.084 0.165 0.231 0.373 0.588 0.93 1.489 2.053

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.