Mouse CLEC7A ELISA Kit PicoKine®

C-type lectin domain family 7 member A or Dectin-1 is a protein that in humans is encoded by the CLEC7A gene. This gene is mapped to 6; 6 F3. CLEC7A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with a partial immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor for a variety of β-1,3-linked and β-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Expression is found on myeloid dendritic cells, monocytes, macrophages and B cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 62 69 69 66 2.87 4.3% Sample 2 330 359 343 341 343 10.35 3.0% Sample 3 1181 1329 1177 1283 1242 65.56 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 62 69 69 66 2.87 4.3% Sample 1 66 62 69 69 66 2.87 4.3% Sample 2 330 359 343 341 343 10.35 3.0% Sample 2 330 359 343 341 343 10.35 3.0% Sample 3 1181 1329 1177 1283 1242 65.56 5.2% Sample 3 1181 1329 1177 1283 1242 65.56 5.2%

C-type lectin domain family 7 member A; Beta-glucan receptor; C-type lectin superfamily member 12; Dendritic cell-associated C-type lectin 1; DC-associated C-type lectin 1; Dectin-1; CD369; Clec7a; Bgr; Clecsf12; Dectin1

Detected in spleen (at protein level) . Highly expressed in dendritic cells, spleen and thymus. Detected in epidermal Langerhans cells. Detected in macrophages, liver and lung.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 330 1181 87 372 1349 Standard deviation 2.77 14.19 56.69 4.26 23.81 91.73 CV (%) 4.2% 4.3% 4.8% 4.9% 6.4% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 330 1181 87 372 1349 Standard deviation 2.77 14.19 56.69 4.26 23.81 91.73 CV (%) 4.2% 4.3% 4.8% 4.9% 6.4% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 66 330 1181 87 372 1349 Mean (pg/ml) 66 330 1181 87 372 1349 Standard deviation 2.77 14.19 56.69 4.26 23.81 91.73 Standard deviation 2.77 14.19 56.69 4.26 23.81 91.73 CV (%) 4.2% 4.3% 4.8% 4.9% 6.4% 6.8% CV (%) 4.2% 4.3% 4.8% 4.9% 6.4% 6.8%

Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.118 0.175 0.29 0.489 0.907 1.814 2.542 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.118 0.175 0.29 0.489 0.907 1.814 2.542 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.118 0.175 0.29 0.489 0.907 1.814 2.542 O.D. 0.051 0.118 0.175 0.29 0.489 0.907 1.814 2.542

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.