Human Tenascin-R ELISA Kit PicoKine®

Tenascin-R is a protein that in humans is encoded by the TNR gene. This gene is mapped to 1q25.1. Tenascin-R (TNR) is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion), TNX (TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The encoded protein is restricted to the central nervous system. The protein may play a role in neurite outgrowth, neural cell adhesion and modulation of sodium channel function. It is a constituent of perineuronal nets.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 250 222 222 221 228 12.27 5.3% Sample 2 1117 1084 1096 1139 1109 20.96 1.8% Sample 3 4237 4064 4513 4218 4258 161.78 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 250 222 222 221 228 12.27 5.3% Sample 1 250 222 222 221 228 12.27 5.3% Sample 2 1117 1084 1096 1139 1109 20.96 1.8% Sample 2 1117 1084 1096 1139 1109 20.96 1.8% Sample 3 4237 4064 4513 4218 4258 161.78 3.7% Sample 3 4237 4064 4513 4218 4258 161.78 3.7%

Axonal Guidance Proteins, Cell Adhesion Proteins, Cytoskeleton/ECM, Developmental Biology, ECM Proteins, Extracellular Matrix, Growth and Development, Nervous System Development, Neurology Process, Neuroscience, Organogenesis, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 250 1117 4237 299 1158 4857 Standard deviation 10.25 63.67 194.90 17.34 79.90 310.85 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 250 1117 4237 299 1158 4857 Standard deviation 10.25 63.67 194.90 17.34 79.90 310.85 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 250 1117 4237 299 1158 4857 Mean (pg/ml) 250 1117 4237 299 1158 4857 Standard deviation 10.25 63.67 194.90 17.34 79.90 310.85 Standard deviation 10.25 63.67 194.90 17.34 79.90 310.85 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4%

Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.076 0.13 0.185 0.292 0.5 0.839 1.496 2.292 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.076 0.13 0.185 0.292 0.5 0.839 1.496 2.292 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.076 0.13 0.185 0.292 0.5 0.839 1.496 2.292 O.D. 0.076 0.13 0.185 0.292 0.5 0.839 1.496 2.292

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.