Human FCRL1 ELISA Kit PicoKine®

Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 40 42 45 42 1.78 4.2% Sample 2 182 167 169 161 169 7.66 4.5% Sample 3 837 797 842 900 844 36.73 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 40 42 45 42 1.78 4.2% Sample 1 42 40 42 45 42 1.78 4.2% Sample 2 182 167 169 161 169 7.66 4.5% Sample 2 182 167 169 161 169 7.66 4.5% Sample 3 837 797 842 900 844 36.73 4.3% Sample 3 837 797 842 900 844 36.73 4.3%

Fc receptor-like protein 1; FcR-like protein 1; FcRL1; Fc receptor homolog 1; FcRH1; IFGP family protein 1; Hifgp1; Immune receptor translocation-associated protein 5; CD307a; FCRL1; FCRH1; IFGP1; IRTA5

Primarily expressed in secondary lymphoid tissues by mature subsets of B-cells. Detected in spleen, lymph node, heart, skeletal muscle, kidney, liver and placenta. Specifically expressed by mature B lineage cells with higher expression in naive versus memory B-cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 182 837 51 193 947 Standard deviation 2.18 9.65 46.04 3.11 12.35 63.45 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 182 837 51 193 947 Standard deviation 2.18 9.65 46.04 3.11 12.35 63.45 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 42 182 837 51 193 947 Mean (pg/ml) 42 182 837 51 193 947 Standard deviation 2.18 9.65 46.04 3.11 12.35 63.45 Standard deviation 2.18 9.65 46.04 3.11 12.35 63.45 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.076 0.15 0.205 0.312 0.52 0.839 1.496 2.392 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.076 0.15 0.205 0.312 0.52 0.839 1.496 2.392 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.076 0.15 0.205 0.312 0.52 0.839 1.496 2.392 O.D. 0.076 0.15 0.205 0.312 0.52 0.839 1.496 2.392

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.