Human CLEC1B ELISA Kit PicoKine®

C-type lectin domain family 1 member B is a protein that in humans is encoded by the CLEC1B gene. This gene is mapped to 12p13.31-p13.2. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 106 107 111 107 2.54 2.3% Sample 2 378 373 365 379 373 5.53 1.4% Sample 3 1786 1782 1704 1930 1800 81.60 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 106 107 111 107 2.54 2.3% Sample 1 104 106 107 111 107 2.54 2.3% Sample 2 378 373 365 379 373 5.53 1.4% Sample 2 378 373 365 379 373 5.53 1.4% Sample 3 1786 1782 1704 1930 1800 81.60 4.5% Sample 3 1786 1782 1704 1930 1800 81.60 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 378 1786 113 418 1824 Standard deviation 4.37 20.03 82.16 5.42 26.75 133.15 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 378 1786 113 418 1824 Standard deviation 4.37 20.03 82.16 5.42 26.75 133.15 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 104 378 1786 113 418 1824 Mean (pg/ml) 104 378 1786 113 418 1824 Standard deviation 4.37 20.03 82.16 5.42 26.75 133.15 Standard deviation 4.37 20.03 82.16 5.42 26.75 133.15 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3%

C-type lectin-like receptor that functions as a platelet receptor for the lymphatic endothelial marker, PDPN. After ligand activation, signals via sequential activation of SRC and SYK tyrosine kinases leading to activation of PLCG2. (Microbial infection) Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (SYK) and initiation of downstream tyrosine phosphorylation events and activation of PLCG2. (Microbial infection) Acts as an attachment factor for Human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.084 0.137 0.25 0.438 0.851 1.626 2.167 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.084 0.137 0.25 0.438 0.851 1.626 2.167 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.084 0.137 0.25 0.438 0.851 1.626 2.167 O.D. 0.035 0.084 0.137 0.25 0.438 0.851 1.626 2.167

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.