Human IL-17F/Interleukin-17F ELISA Kit PicoKine®

Members of the interleukin-17 family, such as IL17F, are involved in the regulation of normal versus aberrant T-cell responses.. It is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 121 134 142 126 130 7.98 6.1% Sample 2 432 464 435 494 456 25.12 5.5% Sample 3 1856 1822 1758 1980 1854 80.80 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 121 134 142 126 130 7.98 6.1% Sample 1 121 134 142 126 130 7.98 6.1% Sample 2 432 464 435 494 456 25.12 5.5% Sample 2 432 464 435 494 456 25.12 5.5% Sample 3 1856 1822 1758 1980 1854 80.80 4.3% Sample 3 1856 1822 1758 1980 1854 80.80 4.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 121 432 1856 123 462 19274 Standard deviation 7.50 28.94 83.52 8.49 32.80 140.67 CV (%) 6.2% 6.7% 4.5% 6.9% 7.1% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 121 432 1856 123 462 19274 Standard deviation 7.50 28.94 83.52 8.49 32.80 140.67 CV (%) 6.2% 6.7% 4.5% 6.9% 7.1% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 121 432 1856 123 462 19274 Mean (pg/ml) 121 432 1856 123 462 19274 Standard deviation 7.50 28.94 83.52 8.49 32.80 140.67 Standard deviation 7.50 28.94 83.52 8.49 32.80 140.67 CV (%) 6.2% 6.7% 4.5% 6.9% 7.1% 7.3% CV (%) 6.2% 6.7% 4.5% 6.9% 7.1% 7.3%

Ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover. Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis. Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.055 0.103 0.162 0.305 0.627 1.201 1.992 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.055 0.103 0.162 0.305 0.627 1.201 1.992 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.055 0.103 0.162 0.305 0.627 1.201 1.992 O.D. 0.035 0.055 0.103 0.162 0.305 0.627 1.201 1.992

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.