Human Nephrin ELISA Kit PicoKine®

Nephrin is a protein necessary for the proper functioning of the renal filtration barrier. It is mapped to 19q13.12. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 216 255 248 232 19.52 8.4% Sample 2 1142 1344 1347 1325 1289 85.57 6.6% Sample 3 4995 4549 4974 5064 4895 202.80 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 216 255 248 232 19.52 8.4% Sample 1 210 216 255 248 232 19.52 8.4% Sample 2 1142 1344 1347 1325 1289 85.57 6.6% Sample 2 1142 1344 1347 1325 1289 85.57 6.6% Sample 3 4995 4549 4974 5064 4895 202.80 4.1% Sample 3 4995 4549 4974 5064 4895 202.80 4.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 1142 4995 287 1167 49998 Standard deviation 8.61 51.39 234.77 19.52 68.85 3049.88 CV (%) 4.1% 4.5% 4.7% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 1142 4995 287 1167 49998 Standard deviation 8.61 51.39 234.77 19.52 68.85 3049.88 CV (%) 4.1% 4.5% 4.7% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 210 1142 4995 287 1167 49998 Mean (pg/ml) 210 1142 4995 287 1167 49998 Standard deviation 8.61 51.39 234.77 19.52 68.85 3049.88 Standard deviation 8.61 51.39 234.77 19.52 68.85 3049.88 CV (%) 4.1% 4.5% 4.7% 6.8% 5.9% 6.1% CV (%) 4.1% 4.5% 4.7% 6.8% 5.9% 6.1%

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.157 0.265 0.475 0.844 1.329 1.935 2.365 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.157 0.265 0.475 0.844 1.329 1.935 2.365 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.157 0.265 0.475 0.844 1.329 1.935 2.365 O.D. 0.033 0.157 0.265 0.475 0.844 1.329 1.935 2.365

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.