Human PSG1 ELISA Kit PicoKine®

The PSG1 is a semi-automatic sniper rifle, Pregnancy-specific beta-1 glycoprotein 1 (PSG-1) is also known as SP1, PSbG1, or B1G1, and is designated CD66f. It is mapped to 19q13.2. The human placenta is a multihormonal endocrine organ that produces hormones, enzymes, and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG, PSG) is a major product of the syncytiotrophoblast, reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women. PSG is a member of the immunoglobulin (Ig) superfamily.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2327 2279 2306 2344 2314 24.27 1.0% Sample 2 9714 10138 10569 10990 10352 476.17 4.5% Sample 3 48035 41237 47332 49070 46418 3054.74 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2327 2279 2306 2344 2314 24.27 1.0% Sample 1 2327 2279 2306 2344 2314 24.27 1.0% Sample 2 9714 10138 10569 10990 10352 476.17 4.5% Sample 2 9714 10138 10569 10990 10352 476.17 4.5% Sample 3 48035 41237 47332 49070 46418 3054.74 6.5% Sample 3 48035 41237 47332 49070 46418 3054.74 6.5%

Pregnancy-specific beta-1-glycoprotein 1; PS-beta-G-1; PSBG-1; Pregnancy-specific glycoprotein 1; CD66 antigen-like family member F; Fetal liver non-specific cross-reactive antigen 1/2; FL-NCA-1/2; PSG95; Pregnancy-specific beta-1 glycoprotein C/D; PS-beta-C/D; CD66f; PSG1; B1G1; PSBG; PSGGA

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2327 9714 48035 2915 11475 48927 Standard deviation 114.02 456.56 1969.44 163.24 585.23 3229.18 CV (%) 4.9% 4.7% 4.1% 5.6% 5.1% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2327 9714 48035 2915 11475 48927 Standard deviation 114.02 456.56 1969.44 163.24 585.23 3229.18 CV (%) 4.9% 4.7% 4.1% 5.6% 5.1% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2327 9714 48035 2915 11475 48927 Mean (pg/ml) 2327 9714 48035 2915 11475 48927 Standard deviation 114.02 456.56 1969.44 163.24 585.23 3229.18 Standard deviation 114.02 456.56 1969.44 163.24 585.23 3229.18 CV (%) 4.9% 4.7% 4.1% 5.6% 5.1% 6.6% CV (%) 4.9% 4.7% 4.1% 5.6% 5.1% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.009 0.085 0.148 0.264 0.516 0.925 1.499 2.007 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.009 0.085 0.148 0.264 0.516 0.925 1.499 2.007 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.009 0.085 0.148 0.264 0.516 0.925 1.499 2.007 O.D. 0.009 0.085 0.148 0.264 0.516 0.925 1.499 2.007

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.