Mouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine®

Cadherin-17 is a protein that in humans is encoded by the CDH17 gene. It is mapped to 4; 4 A1. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 87 79 82 84 4.27 5.0% Sample 2 369 386 427 423 401 24.53 6.1% Sample 3 1459 1399 1340 1383 1395 42.66 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 87 79 82 84 4.27 5.0% Sample 1 90 87 79 82 84 4.27 5.0% Sample 2 369 386 427 423 401 24.53 6.1% Sample 2 369 386 427 423 401 24.53 6.1% Sample 3 1459 1399 1340 1383 1395 42.66 3.0% Sample 3 1459 1399 1340 1383 1395 42.66 3.0%

Highest expression is found in intestine with lower expression in spleen, bone marrow, lung and testis. No expression detected in liver, kidney, heart, brain or skeletal muscle. Expressed in precursor B-cells and myeloid cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 369 1459 91 370 1485 Standard deviation 4.41 19.56 97.75 5.82 21.46 117.32 CV (%) 4.9% 5.3% 6.7% 6.4% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 369 1459 91 370 1485 Standard deviation 4.41 19.56 97.75 5.82 21.46 117.32 CV (%) 4.9% 5.3% 6.7% 6.4% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 369 1459 91 370 1485 Mean (pg/ml) 90 369 1459 91 370 1485 Standard deviation 4.41 19.56 97.75 5.82 21.46 117.32 Standard deviation 4.41 19.56 97.75 5.82 21.46 117.32 CV (%) 4.9% 5.3% 6.7% 6.4% 5.8% 7.9% CV (%) 4.9% 5.3% 6.7% 6.4% 5.8% 7.9%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.049 0.12 0.187 0.306 0.564 0.979 1.483 2.056 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.049 0.12 0.187 0.306 0.564 0.979 1.483 2.056 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.049 0.12 0.187 0.306 0.564 0.979 1.483 2.056 O.D. 0.049 0.12 0.187 0.306 0.564 0.979 1.483 2.056

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.