Human TREM-1 ELISA Kit PicoKine®

Triggering receptor expressed on myeloid cells 1 is a protein that in humans is encoded by the TREM1 gene. It is mapped to 6p21.1. This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 62 57 57 57 2.58 4.5% Sample 2 217 241 248 252 239 13.57 5.6% Sample 3 800 870 776 899 836 50.05 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 62 57 57 57 2.58 4.5% Sample 1 55 62 57 57 57 2.58 4.5% Sample 2 217 241 248 252 239 13.57 5.6% Sample 2 217 241 248 252 239 13.57 5.6% Sample 3 800 870 776 899 836 50.05 5.9% Sample 3 800 870 776 899 836 50.05 5.9%

Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 217 800 58 234 927 Standard deviation 2.59 11.50 44.80 3.89 14.27 63.04 CV (%) 4.7% 5.3% 5.6% 6.7% 6.1% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 217 800 58 234 927 Standard deviation 2.59 11.50 44.80 3.89 14.27 63.04 CV (%) 4.7% 5.3% 5.6% 6.7% 6.1% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 217 800 58 234 927 Mean (pg/ml) 55 217 800 58 234 927 Standard deviation 2.59 11.50 44.80 3.89 14.27 63.04 Standard deviation 2.59 11.50 44.80 3.89 14.27 63.04 CV (%) 4.7% 5.3% 5.6% 6.7% 6.1% 6.8% CV (%) 4.7% 5.3% 5.6% 6.7% 6.1% 6.8%

Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.06 0.097 0.17 0.354 0.653 1.188 2.446 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.06 0.097 0.17 0.354 0.653 1.188 2.446 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.06 0.097 0.17 0.354 0.653 1.188 2.446 O.D. 0.015 0.06 0.097 0.17 0.354 0.653 1.188 2.446

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.