Human Pregnancy Zone Protein/PZP ELISA Kit PicoKine®

Pregnancy zone protein (PZP) is a protein which in humans is encoded by the PZP gene on chromosome 12. It is mapped to 12p13.31. The protein encoded by this gene is highly expressed in late-pregnancy serum and is similar in structure to alpha-2-macroglobulin. The encoded protein, which acts as a homotetramer, inhibits the activity of all four classes of proteinases. This protein contains cleavage sites for several proteinases. Upon binding of a proteinase, the conformation of this protein changes to trap the proteinase, limiting its activity. This protein appears to be elevated in the sera of presymptomatic Alzheimer's disease patients.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 252 219 220 232 13.91 5.9% Sample 2 952 1062 957 1054 1006 51.85 5.1% Sample 3 4494 4533 4059 4186 4318 201.06 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 252 219 220 232 13.91 5.9% Sample 1 240 252 219 220 232 13.91 5.9% Sample 2 952 1062 957 1054 1006 51.85 5.1% Sample 2 952 1062 957 1054 1006 51.85 5.1% Sample 3 4494 4533 4059 4186 4318 201.06 4.6% Sample 3 4494 4533 4059 4186 4318 201.06 4.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 952 4494 289 1147 4732 Standard deviation 10.08 58.07 292.11 15.61 72.26 359.63 CV (%) 4.2% 6.1% 6.5% 5.4% 6.3% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 952 4494 289 1147 4732 Standard deviation 10.08 58.07 292.11 15.61 72.26 359.63 CV (%) 4.2% 6.1% 6.5% 5.4% 6.3% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 240 952 4494 289 1147 4732 Mean (pg/ml) 240 952 4494 289 1147 4732 Standard deviation 10.08 58.07 292.11 15.61 72.26 359.63 Standard deviation 10.08 58.07 292.11 15.61 72.26 359.63 CV (%) 4.2% 6.1% 6.5% 5.4% 6.3% 7.6% CV (%) 4.2% 6.1% 6.5% 5.4% 6.3% 7.6%

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced) . Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.132 0.212 0.335 0.719 1.161 1.868 2.462 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.132 0.212 0.335 0.719 1.161 1.868 2.462 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.132 0.212 0.335 0.719 1.161 1.868 2.462 O.D. 0.04 0.132 0.212 0.335 0.719 1.161 1.868 2.462

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.