Rat SCGB3A1 ELISA Kit PicoKine®

Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. It is mapped to 10q21. Members of the secretoglobin family, such as SCGB3A1, are secreted proteins of about 10 kD that are found in high concentrations in fluids of lung, lacrimal gland, salivary gland, prostate, uterus, and other tissues.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 34 33 32 32 1.47 4.5% Sample 2 176 163 162 173 168 6.10 3.6% Sample 3 389 365 413 429 399 24.24 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 34 33 32 32 1.47 4.5% Sample 1 30 34 33 32 32 1.47 4.5% Sample 2 176 163 162 173 168 6.10 3.6% Sample 2 176 163 162 173 168 6.10 3.6% Sample 3 389 365 413 429 399 24.24 6.0% Sample 3 389 365 413 429 399 24.24 6.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 176 389 31 184 406 Standard deviation 1.41 9.15 22.17 1.95 9.94 28.83 CV (%) 4.7% 5.2% 5.7% 6.3% 5.4% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 176 389 31 184 406 Standard deviation 1.41 9.15 22.17 1.95 9.94 28.83 CV (%) 4.7% 5.2% 5.7% 6.3% 5.4% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 176 389 31 184 406 Mean (pg/ml) 30 176 389 31 184 406 Standard deviation 1.41 9.15 22.17 1.95 9.94 28.83 Standard deviation 1.41 9.15 22.17 1.95 9.94 28.83 CV (%) 4.7% 5.2% 5.7% 6.3% 5.4% 7.1% CV (%) 4.7% 5.2% 5.7% 6.3% 5.4% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.03 0.117 0.199 0.323 0.59 0.939 1.654 2.28 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.03 0.117 0.199 0.323 0.59 0.939 1.654 2.28 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.03 0.117 0.199 0.323 0.59 0.939 1.654 2.28 O.D. 0.03 0.117 0.199 0.323 0.59 0.939 1.654 2.28

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.