Human ANGPTL6 ELISA Kit PicoKine®

Angiopoietin-like 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain and a fibrinogen-like domain. It is mapped to 19p13.2. ANGPTL6 is highly expressed in hepatocytes and is detectable in serum. Within the liver, secreted ANGPTL6 inhibits gluconeogenesis and promotes insulin sensitivity and energy expenditure. Circulating ANGPTL6 promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. An integrin--binding RGD motif located within the fibrinogen--like domain of ANGPTL6, enables it to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. Elevated levels of ANGPTL6 in serum are associated with metabolic syndrome, diabetes, and pre-eclampsia. Serum ANGPTL6 levels are also elevated during obesity. ANGPTL6 is reported to promote the survival and expansion of hematopoietic stem cells. ANGPTL6 is produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature human ANGPTL6 shares 75% sequence identity with both mouse and rat ANGPTL6.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 50 47 55 49 3.50 7.1% Sample 2 215 229 200 212 214 10.31 4.8% Sample 3 874 845 905 924 887 30.10 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 50 47 55 49 3.50 7.1% Sample 1 46 50 47 55 49 3.50 7.1% Sample 2 215 229 200 212 214 10.31 4.8% Sample 2 215 229 200 212 214 10.31 4.8% Sample 3 874 845 905 924 887 30.10 3.3% Sample 3 874 845 905 924 887 30.10 3.3%

Angiopoietin-related protein 6; Angiopoietin-like protein 6; Angiopoietin-related growth factor; Angiopoietin-related protein 5; ANGPTL6; AGF; ARP5; UNQ152; PRO178

Angiogenesis, Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 215 874 49 221 896 Standard deviation 11.24 68.76 245.93 10.90 79.04 332.32 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 215 874 49 221 896 Standard deviation 11.24 68.76 245.93 10.90 79.04 332.32 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 215 874 49 221 896 Mean (pg/ml) 46 215 874 49 221 896 Standard deviation 11.24 68.76 245.93 10.90 79.04 332.32 Standard deviation 11.24 68.76 245.93 10.90 79.04 332.32 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4%

May play a role in the wound healing process. May promote epidermal proliferation, remodeling and regeneration. May promote the chemotactic activity of endothelial cells and induce neovascularization. May counteract high-fat diet-induced obesity and related insulin resistance through increased energy expenditure.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.176 0.248 0.365 0.627 1.0165 1.648 2.73 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.176 0.248 0.365 0.627 1.0165 1.648 2.73 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.176 0.248 0.365 0.627 1.0165 1.648 2.73 O.D. 0.056 0.176 0.248 0.365 0.627 1.0165 1.648 2.73

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.