Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine®

Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. It is mapped to 8q32. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G, chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K , a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 54 55 54 55 1.63 2.9% Sample 2 227 223 217 225 223 3.74 1.6% Sample 3 919 937 964 972 948 21.17 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 54 55 54 55 1.63 2.9% Sample 1 58 54 55 54 55 1.63 2.9% Sample 2 227 223 217 225 223 3.74 1.6% Sample 2 227 223 217 225 223 3.74 1.6% Sample 3 919 937 964 972 948 21.17 2.2% Sample 3 919 937 964 972 948 21.17 2.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 227 919 59 236 947 Standard deviation 3.02 12.03 43.19 3.60 17.46 48.30 CV (%) 5.2% 5.3% 4.7% 6.1% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 227 919 59 236 947 Standard deviation 3.02 12.03 43.19 3.60 17.46 48.30 CV (%) 5.2% 5.3% 4.7% 6.1% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 58 227 919 59 236 947 Mean (pg/ml) 58 227 919 59 236 947 Standard deviation 3.02 12.03 43.19 3.60 17.46 48.30 Standard deviation 3.02 12.03 43.19 3.60 17.46 48.30 CV (%) 5.2% 5.3% 4.7% 6.1% 7.4% 5.1% CV (%) 5.2% 5.3% 4.7% 6.1% 7.4% 5.1%

Coreceptor for SEMA3A and SEMA3F. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance in the developing nervous system. Regulates the migration of sympathetic neurons, but not of neural crest precursors. Required for normal dendrite spine morphology in pyramidal neurons. May play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.103 0.16 0.251 0.426 0.749 1.205 2.104 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.103 0.16 0.251 0.426 0.749 1.205 2.104 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.103 0.16 0.251 0.426 0.749 1.205 2.104 O.D. 0.037 0.103 0.16 0.251 0.426 0.749 1.205 2.104

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.