Mouse SEMA3F/Semaphorin-3F ELISA Kit PicoKine®

Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. It is mapped to 9; 9 F1. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G, chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K , a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 70 64 60 63 4.32 6.8% Sample 2 231 224 225 261 235 15.10 6.4% Sample 3 896 856 928 913 898 26.89 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 70 64 60 63 4.32 6.8% Sample 1 59 70 64 60 63 4.32 6.8% Sample 2 231 224 225 261 235 15.10 6.4% Sample 2 231 224 225 261 235 15.10 6.4% Sample 3 896 856 928 913 898 26.89 2.9% Sample 3 896 856 928 913 898 26.89 2.9%

Expressed ubiquitously in adulthood. During embryogenesis, expressed in subregions of the central nervous system and various other tissues like skin, kidney, lung and intestine.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 231 896 61 245 907 Standard deviation 3.48 10.16 56.45 4.03 14.21 68.03 CV (%) 5.9% 4.4% 6.3% 6.6% 5.8% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 231 896 61 245 907 Standard deviation 3.48 10.16 56.45 4.03 14.21 68.03 CV (%) 5.9% 4.4% 6.3% 6.6% 5.8% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 231 896 61 245 907 Mean (pg/ml) 59 231 896 61 245 907 Standard deviation 3.48 10.16 56.45 4.03 14.21 68.03 Standard deviation 3.48 10.16 56.45 4.03 14.21 68.03 CV (%) 5.9% 4.4% 6.3% 6.6% 5.8% 7.5% CV (%) 5.9% 4.4% 6.3% 6.6% 5.8% 7.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.12 0.186 0.286 0.476 0.828 1.398 2.201 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.12 0.186 0.286 0.476 0.828 1.398 2.201 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.12 0.186 0.286 0.476 0.828 1.398 2.201 O.D. 0.054 0.12 0.186 0.286 0.476 0.828 1.398 2.201

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.