Human GAS6 ELISA Kit PicoKine®

Growth arrest-specific 6, also known as GAS6, is a human gene coding for the Gas6 protein. It is mapped to 13q34. This gene encodes a gamma-carboxyglutamic acid (Gla) -containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. Gas6 is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 92 101 100 98 3.53 3.6% Sample 2 434 424 411 459 432 17.59 4.0% Sample 3 1403 1407 1524 1413 1436 50.49 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 92 101 100 98 3.53 3.6% Sample 1 99 92 101 100 98 3.53 3.6% Sample 2 434 424 411 459 432 17.59 4.0% Sample 2 434 424 411 459 432 17.59 4.0% Sample 3 1403 1407 1524 1413 1436 50.49 3.5% Sample 3 1403 1407 1524 1413 1436 50.49 3.5%

Plasma. Isoform 1 and isoform 2 are widely expressed, isoform 1 being expressed at higher levels than isoform 2 in most tissues. Isoform 2 is the predominant form in spleen.

Atherosclerosis, Cardiovascular, Hematopoietic Progenitors, Mesenchymal Stem Cells, Myeloid, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Stem Cells, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 434 1403 119 482 1746 Standard deviation 4.26 28.21 65.94 7.38 33.26 123.97 CV (%) 4.3% 6.5% 4.7% 6.2% 6.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 434 1403 119 482 1746 Standard deviation 4.26 28.21 65.94 7.38 33.26 123.97 CV (%) 4.3% 6.5% 4.7% 6.2% 6.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 99 434 1403 119 482 1746 Mean (pg/ml) 99 434 1403 119 482 1746 Standard deviation 4.26 28.21 65.94 7.38 33.26 123.97 Standard deviation 4.26 28.21 65.94 7.38 33.26 123.97 CV (%) 4.3% 6.5% 4.7% 6.2% 6.9% 7.1% CV (%) 4.3% 6.5% 4.7% 6.2% 6.9% 7.1%

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. (Microbial infection) Can bridges virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry. Plays a role in Dengue cell entry by apoptotic mimicry. Plays a role in Vaccinia virus cell entry by apoptotic mimicry. Plays a role in ebolavirus and marburgvirus cell entry by apoptotic mimicry.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.056 0.138 0.238 0.365 0.657 1.151 1.648 2.23 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.056 0.138 0.238 0.365 0.657 1.151 1.648 2.23 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.056 0.138 0.238 0.365 0.657 1.151 1.648 2.23 O.D. 0.056 0.138 0.238 0.365 0.657 1.151 1.648 2.23

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.