Human FUT8 ELISA Kit PicoKine®

Human FUT8 ELISA Kit PicoKine® (96 Tests) . Quantitate Human FUT8 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Alpha- (1,6) -fucosyltransferase is an enzyme that in humans is encoded by the FUT8 gene. It is mapped to 14q23.3. This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 281 268 268 273 5.84 2.1% Sample 2 1061 1124 1190 1141 1129 46.13 4.0% Sample 3 4415 4940 4601 4553 4627 193.04 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 281 268 268 273 5.84 2.1% Sample 1 278 281 268 268 273 5.84 2.1% Sample 2 1061 1124 1190 1141 1129 46.13 4.0% Sample 2 1061 1124 1190 1141 1129 46.13 4.0% Sample 3 4415 4940 4601 4553 4627 193.04 4.1% Sample 3 4415 4940 4601 4553 4627 193.04 4.1%

Synonyms

Alpha- (1,6) -fucosyltransferase; Alpha1-6FucT; Fucosyltransferase 8; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; GDP-fucose--glycoprotein fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; FUT8

Gene Name

FUT8

Gene ID

2530

UniProt

Q9BYC5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R31-K575

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin or EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1061 4415 296 1147 4781 Standard deviation 13.34 67.90 198.68 20.13 83.73 320.33 CV (%) 4.8% 6.4% 4.5% 6.8% 7.3% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1061 4415 296 1147 4781 Standard deviation 13.34 67.90 198.68 20.13 83.73 320.33 CV (%) 4.8% 6.4% 4.5% 6.8% 7.3% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1061 4415 296 1147 4781 Mean (pg/ml) 278 1061 4415 296 1147 4781 Standard deviation 13.34 67.90 198.68 20.13 83.73 320.33 Standard deviation 13.34 67.90 198.68 20.13 83.73 320.33 CV (%) 4.8% 6.4% 4.5% 6.8% 7.3% 6.7% CV (%) 4.8% 6.4% 4.5% 6.8% 7.3% 6.7%

Sensitivity

<50 pg/mL

Function

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human FUT8

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fucosyltransferase 8

Subcellular Location

Golgi stack membrane. Single-pass type II membrane protein.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.157 0.224 0.365 0.6 1.128 1.551 2.058 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.157 0.224 0.365 0.6 1.128 1.551 2.058 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.157 0.224 0.365 0.6 1.128 1.551 2.058 O.D. 0.051 0.157 0.224 0.365 0.6 1.128 1.551 2.058

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.