Rat STC2/Stanniocalcin 2 ELISA Kit PicoKine®

Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 10q12. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers. STC2 has opposite effects on calcium and phosphate homeostasis as compared to Stc1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 309 293 279 275 289 13.34 4.6% Sample 2 1116 1201 1281 1231 1207 59.93 4.9% Sample 3 4854 4943 4684 4661 4785 117.58 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 309 293 279 275 289 13.34 4.6% Sample 1 309 293 279 275 289 13.34 4.6% Sample 2 1116 1201 1281 1231 1207 59.93 4.9% Sample 2 1116 1201 1281 1231 1207 59.93 4.9% Sample 3 4854 4943 4684 4661 4785 117.58 2.4% Sample 3 4854 4943 4684 4661 4785 117.58 2.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 309 1116 4854 311 1189 4887 Standard deviation 12.98 50.22 276.68 15.24 68.96 307.88 CV (%) 4.2% 4.5% 5.7% 4.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 309 1116 4854 311 1189 4887 Standard deviation 12.98 50.22 276.68 15.24 68.96 307.88 CV (%) 4.2% 4.5% 5.7% 4.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 309 1116 4854 311 1189 4887 Mean (pg/ml) 309 1116 4854 311 1189 4887 Standard deviation 12.98 50.22 276.68 15.24 68.96 307.88 Standard deviation 12.98 50.22 276.68 15.24 68.96 307.88 CV (%) 4.2% 4.5% 5.7% 4.9% 5.8% 6.3% CV (%) 4.2% 4.5% 5.7% 4.9% 5.8% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.139 0.224 0.395 0.755 1.125 1.796 2.349 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.139 0.224 0.395 0.755 1.125 1.796 2.349 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.139 0.224 0.395 0.755 1.125 1.796 2.349 O.D. 0.045 0.139 0.224 0.395 0.755 1.125 1.796 2.349

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.