Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine®

Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 11; 11 A4. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 253 236 226 240 10.35 4.3% Sample 2 1255 1282 1191 1248 1244 33.12 2.6% Sample 3 4504 4761 4782 5438 4871 345.03 7.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 253 236 226 240 10.35 4.3% Sample 1 247 253 236 226 240 10.35 4.3% Sample 2 1255 1282 1191 1248 1244 33.12 2.6% Sample 2 1255 1282 1191 1248 1244 33.12 2.6% Sample 3 4504 4761 4782 5438 4871 345.03 7.0% Sample 3 4504 4761 4782 5438 4871 345.03 7.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1255 4504 284 1264 4728 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1255 4504 284 1264 4728 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 1255 4504 284 1264 4728 Mean (pg/ml) 247 1255 4504 284 1264 4728 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.062 0.101 0.192 0.446 0.712 1.257 1.95 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.062 0.101 0.192 0.446 0.712 1.257 1.95 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.062 0.101 0.192 0.446 0.712 1.257 1.95 O.D. 0.021 0.062 0.101 0.192 0.446 0.712 1.257 1.95

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.