Mouse STC2/Stanniocalcin 2 ELISA Kit PicoKine®

Mouse STC2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse STC2 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 11; 11 A4. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 253 236 226 240 10.35 4.3% Sample 2 1255 1282 1191 1248 1244 33.12 2.6% Sample 3 4504 4761 4782 5438 4871 345.03 7.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 253 236 226 240 10.35 4.3% Sample 1 247 253 236 226 240 10.35 4.3% Sample 2 1255 1282 1191 1248 1244 33.12 2.6% Sample 2 1255 1282 1191 1248 1244 33.12 2.6% Sample 3 4504 4761 4782 5438 4871 345.03 7.0% Sample 3 4504 4761 4782 5438 4871 345.03 7.0%

Synonyms

Stanniocalcin-2; STC-2; Stc2

Gene Name

Stc2

Gene ID

20856

UniProt

O88452

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T25-R296

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.

Applications

ELISA

Field of Research

Calcium Signaling, Mesenchymal Stem Cells, Secreted Molecules, Signal Transduction, Signaling Pathway, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1255 4504 284 1264 4728 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1255 4504 284 1264 4728 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 1255 4504 284 1264 4728 Mean (pg/ml) 247 1255 4504 284 1264 4728 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3%

Sensitivity

<50 pg/mL

Function

Has an anti-hypocalcemic action on calcium and phosphate homeostasis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse STC2

Anticoagulant

Heparin

Gene Name Synonym

Stanniocalcin 2

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.062 0.101 0.192 0.446 0.712 1.257 1.95 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.062 0.101 0.192 0.446 0.712 1.257 1.95 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.062 0.101 0.192 0.446 0.712 1.257 1.95 O.D. 0.021 0.062 0.101 0.192 0.446 0.712 1.257 1.95

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.