Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine®

Human STC2 / Stanniocalcin 2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human STC2 in cell culture supernatants and serum. Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 5q35.2. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 9 10 9 10 9 0.50 5.5% Sample 2 71 72 61 64 67 4.63 6.9% Sample 3 207 217 226 208 214 7.69 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 9 10 9 10 9 0.50 5.5% Sample 1 9 10 9 10 9 0.50 5.5% Sample 2 71 72 61 64 67 4.63 6.9% Sample 2 71 72 61 64 67 4.63 6.9% Sample 3 207 217 226 208 214 7.69 3.5% Sample 3 207 217 226 208 214 7.69 3.5%

Synonyms

Stanniocalcin-2; STC-2; Stanniocalcin-related protein; STC-related protein; STCRP; STC2

Gene Name

STC2

Gene ID

8614

UniProt

O76061

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T25-R302

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leukocytes.

Applications

ELISA

Field of Research

Calcium Signaling, Mesenchymal Stem Cells, Secreted Molecules, Signal Transduction, Signaling Pathway, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 9 71 207 12 75 219 Standard deviation 0.38 3.62 11.39 0.82 4.43 13.36 CV (%) 4.2% 5.1% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 9 71 207 12 75 219 Standard deviation 0.38 3.62 11.39 0.82 4.43 13.36 CV (%) 4.2% 5.1% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 9 71 207 12 75 219 Mean (pg/ml) 9 71 207 12 75 219 Standard deviation 0.38 3.62 11.39 0.82 4.43 13.36 Standard deviation 0.38 3.62 11.39 0.82 4.43 13.36 CV (%) 4.2% 5.1% 5.5% 6.8% 5.9% 6.1% CV (%) 4.2% 5.1% 5.5% 6.8% 5.9% 6.1%

Sensitivity

<5 pg/mL

Function

Has an anti-hypocalcemic action on calcium and phosphate homeostasis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human STC2

Gene Name Synonym

Stanniocalcin 2

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.027 0.111 0.195 0.346 0.614 1.04 1.697 2.255 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.027 0.111 0.195 0.346 0.614 1.04 1.697 2.255 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.027 0.111 0.195 0.346 0.614 1.04 1.697 2.255 O.D. 0.027 0.111 0.195 0.346 0.614 1.04 1.697 2.255

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.