Human Serpin A12 ELISA Kit PicoKine®

Human Serpin A12 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SERPINA12 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene is mapped to 14q32.13.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 215 232 220 219 7.62 3.4% Sample 2 1109 1215 1235 1149 1177 50.53 4.2% Sample 3 5465 6356 5987 5976 5946 317.03 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 215 232 220 219 7.62 3.4% Sample 1 212 215 232 220 219 7.62 3.4% Sample 2 1109 1215 1235 1149 1177 50.53 4.2% Sample 2 1109 1215 1235 1149 1177 50.53 4.2% Sample 3 5465 6356 5987 5976 5946 317.03 5.3% Sample 3 5465 6356 5987 5976 5946 317.03 5.3%

Synonyms

Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12

Gene Name

SERPINA12

Gene ID

145264

UniProt

Q8IW75

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-K414

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Expressed in visceral adipose tissues.

Applications

ELISA

Field of Research

Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Diabetes, Diabetes-associated, Growth Factors/Hormones, Heart Disease, Metabolism, Neurology Process, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 1109 5465 232 1235 6154 Standard deviation 11.24 68.76 245.93 10.90 79.04 332.32 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 1109 5465 232 1235 6154 Standard deviation 11.24 68.76 245.93 10.90 79.04 332.32 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 212 1109 5465 232 1235 6154 Mean (pg/ml) 212 1109 5465 232 1235 6154 Standard deviation 11.24 68.76 245.93 10.90 79.04 332.32 Standard deviation 11.24 68.76 245.93 10.90 79.04 332.32 CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4% CV (%) 5.3% 6.2% 4.5% 4.7% 6.4% 5.4%

Sensitivity

<50 pg/mL

Function

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Serpin A12

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Serpin family A member 12

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.026 0.067 0.167 0.361 0.87 1.803 2.639 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.026 0.067 0.167 0.361 0.87 1.803 2.639 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.026 0.067 0.167 0.361 0.87 1.803 2.639 O.D. 0.009 0.026 0.067 0.167 0.361 0.87 1.803 2.639

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.