Human IL-32/Interleukin-32 ELISA Kit PicoKine®

Human IL-32 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL32 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 32 (Il32) is a protein that in humans is encoded by the IL32 gene. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 80 89 77 79 5.88 7.4% Sample 2 499 486 505 477 491 10.94 2.2% Sample 3 1288 1381 1358 1225 1313 61.27 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 80 89 77 79 5.88 7.4% Sample 1 73 80 89 77 79 5.88 7.4% Sample 2 499 486 505 477 491 10.94 2.2% Sample 2 499 486 505 477 491 10.94 2.2% Sample 3 1288 1381 1358 1225 1313 61.27 4.6% Sample 3 1288 1381 1358 1225 1313 61.27 4.6%

Synonyms

Interleukin-32; IL-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; IL32; NK4; TAIF

Gene Name

IL32

Gene ID

9235

UniProt

P24001

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A31-K234

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells.

Applications

ELISA

Field of Research

Autoimmune, Cancer, Cytokines, Immune System Diseases, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 499 1288 88 523 1488 Standard deviation 3.58 26.45 86.30 5.63 25.10 87.79 CV (%) 4.9% 5.3% 6.7% 6.4% 4.8% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 499 1288 88 523 1488 Standard deviation 3.58 26.45 86.30 5.63 25.10 87.79 CV (%) 4.9% 5.3% 6.7% 6.4% 4.8% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 73 499 1288 88 523 1488 Mean (pg/ml) 73 499 1288 88 523 1488 Standard deviation 3.58 26.45 86.30 5.63 25.10 87.79 Standard deviation 3.58 26.45 86.30 5.63 25.10 87.79 CV (%) 4.9% 5.3% 6.7% 6.4% 4.8% 5.9% CV (%) 4.9% 5.3% 6.7% 6.4% 4.8% 5.9%

Sensitivity

<10 pg/mL

Function

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human IL-32

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 32

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.166 0.249 0.38 0.671 1.241 1.992 2.492 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.166 0.249 0.38 0.671 1.241 1.992 2.492 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.166 0.249 0.38 0.671 1.241 1.992 2.492 O.D. 0.051 0.166 0.249 0.38 0.671 1.241 1.992 2.492

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.