Mouse CRELD2 ELISA Kit PicoKine®

Mouse CRELD2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Creld2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cysteine-rich with EGF-like domain protein 2 is a protein that in humans is encoded by the CRELD2 gene. It is mapped to 15; 15 E3. CRELD2 was identified by searching a sequence database using CRELD1 as the query. Like CRELD1, with which it shares 38% amino acid identity, the deduced 321-amino acid CRELD2 protein contains EGF-like repeats and a highly conserved domain rich in glutamic acid and tryptophan (WE domain) . The major distinction between CRELD1 and CRELD2 is that CRELD1 has 2 type III transmembrane domains in its C terminus, while CRELD2 has none. CRELD2 shares 67% and 69% amino acid identity with its Chinese hamster and mouse homologs, respectively.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 214 193 191 207 201 9.60 4.7% Sample 2 1244 1136 1076 1247 1175 72.91 6.2% Sample 3 5866 4866 5133 5509 5343 378.38 7.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 214 193 191 207 201 9.60 4.7% Sample 1 214 193 191 207 201 9.60 4.7% Sample 2 1244 1136 1076 1247 1175 72.91 6.2% Sample 2 1244 1136 1076 1247 1175 72.91 6.2% Sample 3 5866 4866 5133 5509 5343 378.38 7.0% Sample 3 5866 4866 5133 5509 5343 378.38 7.0%

Synonyms

Cysteine-rich with EGF-like domain protein 2; Creld2

Gene Name

Creld2

Gene ID

76737

UniProt

Q9CYA0

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S23-L350

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Ligand-Gated Ion Channels, Neuroscience, Neurotransmission, Receptors / Channels

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 214 1244 5866 219 1247 6512 Standard deviation 10.06 65.93 328.50 14.67 63.60 312.58 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 214 1244 5866 219 1247 6512 Standard deviation 10.06 65.93 328.50 14.67 63.60 312.58 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 214 1244 5866 219 1247 6512 Mean (pg/ml) 214 1244 5866 219 1247 6512 Standard deviation 10.06 65.93 328.50 14.67 63.60 312.58 Standard deviation 10.06 65.93 328.50 14.67 63.60 312.58 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8%

Sensitivity

<50 pg/mL

Function

May regulate transport of alpha4-beta2 neuronal acetylcholine receptor.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CRELD2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cysteine-rich with EGF-like domains 2

Subcellular Location

Endoplasmic reticulum. Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.087 0.15 0.249 0.447 0.752 1.243 1.958 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.087 0.15 0.249 0.447 0.752 1.243 1.958 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.087 0.15 0.249 0.447 0.752 1.243 1.958 O.D. 0.025 0.087 0.15 0.249 0.447 0.752 1.243 1.958

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.