Human CD300A ELISA Kit PicoKine®

CD300A (Cluster of Differentiation 300A) is a human gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 362 424 405 365 389 26.39 6.7% Sample 2 1768 1661 1621 1762 1703 63.62 3.7% Sample 3 9786 8596 9892 9589 9465 513.78 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 362 424 405 365 389 26.39 6.7% Sample 1 362 424 405 365 389 26.39 6.7% Sample 2 1768 1661 1621 1762 1703 63.62 3.7% Sample 2 1768 1661 1621 1762 1703 63.62 3.7% Sample 3 9786 8596 9892 9589 9465 513.78 5.4% Sample 3 9786 8596 9892 9589 9465 513.78 5.4%

CMRF35-like molecule 8; CLM-8; CD300 antigen-like family member A; CMRF-35-H9; CMRF35-H9; CMRF35-H; IRC1/IRC2; Immunoglobulin superfamily member 12; IgSF12; Inhibitory receptor protein 60; IRp60; NK inhibitory receptor; CD300a; CD300A; CMRF35H; IGSF12; HS

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 362 1768 9786 405 1772 9892 Standard deviation 218.82 111.38 469.73 19.85 131.13 524.28 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 362 1768 9786 405 1772 9892 Standard deviation 218.82 111.38 469.73 19.85 131.13 524.28 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 362 1768 9786 405 1772 9892 Mean (pg/ml) 362 1768 9786 405 1772 9892 Standard deviation 218.82 111.38 469.73 19.85 131.13 524.28 Standard deviation 218.82 111.38 469.73 19.85 131.13 524.28 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3%

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but no

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.117 0.18 0.306 0.517 0.883 1.64 2.758 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.117 0.18 0.306 0.517 0.883 1.64 2.758 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.117 0.18 0.306 0.517 0.883 1.64 2.758 O.D. 0.046 0.117 0.18 0.306 0.517 0.883 1.64 2.758

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.