Human CRELD2 ELISA Kit PicoKine®

Human CRELD2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CRELD2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cysteine-rich with EGF-like domain protein 2 is a protein that in humans is encoded by the CRELD2 gene. It is mapped to 22q13.33. CRELD2 was identified by searching a sequence database using CRELD1 as the query. Like CRELD1, with which it shares 38% amino acid identity, the deduced 321-amino acid CRELD2 protein contains EGF-like repeats and a highly conserved domain rich in glutamic acid and tryptophan (WE domain) . The major distinction between CRELD1 and CRELD2 is that CRELD1 has 2 type III transmembrane domains in its C terminus, while CRELD2 has none. CRELD2 shares 67% and 69% amino acid identity with its Chinese hamster and mouse homologs, respectively.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 153 154 147 158 153 3.93 2.5% Sample 2 696 700 750 711 714 21.35 2.9% Sample 3 3699 3606 3621 3798 3681 76.22 2.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 153 154 147 158 153 3.93 2.5% Sample 1 153 154 147 158 153 3.93 2.5% Sample 2 696 700 750 711 714 21.35 2.9% Sample 2 696 700 750 711 714 21.35 2.9% Sample 3 3699 3606 3621 3798 3681 76.22 2.0% Sample 3 3699 3606 3621 3798 3681 76.22 2.0%

Synonyms

Cysteine-rich with EGF-like domain protein 2; CRELD2; UNQ185/PRO211

Gene Name

CRELD2

Gene ID

79174

UniProt

Q6UXH1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A25-L353

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Ubiquitously expressed.

Applications

ELISA

Field of Research

Cell Biology, Ligand-Gated Ion Channels, Neuroscience, Neurotransmission, Receptors / Channels

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 153 696 3699 209 874 4132 Standard deviation 6.43 42.46 240.44 11.29 37.58 231.39 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 153 696 3699 209 874 4132 Standard deviation 6.43 42.46 240.44 11.29 37.58 231.39 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 153 696 3699 209 874 4132 Mean (pg/ml) 153 696 3699 209 874 4132 Standard deviation 6.43 42.46 240.44 11.29 37.58 231.39 Standard deviation 6.43 42.46 240.44 11.29 37.58 231.39 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6%

Sensitivity

<50 pg/mL

Function

May regulate transport of alpha4-beta2 neuronal acetylcholine receptor.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CRELD2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cysteine rich with EGF like domains 2

Subcellular Location

Secreted. Endoplasmic reticulum.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.169 0.28 0.471 0.805 1.403 2.042 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.169 0.28 0.471 0.805 1.403 2.042 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.169 0.28 0.471 0.805 1.403 2.042 O.D. 0.045 0.111 0.169 0.28 0.471 0.805 1.403 2.042

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.