Human CILP ELISA Kit PicoKine®

Cartilage intermediate layer protein 1 is a protein that in humans is encoded by the CILP gene. It is mapped to 15q22.31. Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 123 122 110 115 117 5.31 4.5% Sample 2 719 692 671 628 677 33.26 4.9% Sample 3 2659 2437 2461 2706 2565 118.23 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 123 122 110 115 117 5.31 4.5% Sample 1 123 122 110 115 117 5.31 4.5% Sample 2 719 692 671 628 677 33.26 4.9% Sample 2 719 692 671 628 677 33.26 4.9% Sample 3 2659 2437 2461 2706 2565 118.23 4.6% Sample 3 2659 2437 2461 2706 2565 118.23 4.6%

Specifically expressed in cartilage. Localizes in the intermediates layer of articular cartilage but neither in the superficial nor in the deepest regions. Specifically and highly expressed in intervertebral disk tissue. Expression increases with aging in

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 719 2659 143 742 2706 Standard deviation 6.52 35.23 162.20 9.44 41.55 148.83 CV (%) 5.3% 4.9% 6.1% 6.6% 5.6% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 719 2659 143 742 2706 Standard deviation 6.52 35.23 162.20 9.44 41.55 148.83 CV (%) 5.3% 4.9% 6.1% 6.6% 5.6% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 123 719 2659 143 742 2706 Mean (pg/ml) 123 719 2659 143 742 2706 Standard deviation 6.52 35.23 162.20 9.44 41.55 148.83 Standard deviation 6.52 35.23 162.20 9.44 41.55 148.83 CV (%) 5.3% 4.9% 6.1% 6.6% 5.6% 5.5% CV (%) 5.3% 4.9% 6.1% 6.6% 5.6% 5.5%

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.027 0.055 0.101 0.195 0.346 0.614 1.04 1.897 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.027 0.055 0.101 0.195 0.346 0.614 1.04 1.897 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.027 0.055 0.101 0.195 0.346 0.614 1.04 1.897 O.D. 0.027 0.055 0.101 0.195 0.346 0.614 1.04 1.897

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.